abstract：:Influenza viral complementary RNA (cRNA), i.e., viral mRNA was radioactive when purified from the cytoplasmic fraction of cordycepin-treated canine kidney cells that were incubated with [methyl-3H]methionine during infection. Approximately 55 to 60% of the methyl-3H radioactivity was in internal N6-methyladenosine, a ...

journal_title：Journal of virology

authors： Krug RM,Morgan MA,Shatkin AJ

更新日期：1976-10-01 00:00:00

abstract：:An extensive genetic map of coliphage P1 has been constructed for 113 amber mutants, using primarily a modification of the conventional complementation spot test. These spot tests failed to classify the mutants into cistrons, but when they were quantitated they permitted assignment of the mutants into 10 linkage clust...

journal_title：Journal of virology

authors： Walker DH Jr,Walker JT

更新日期：1976-10-01 00:00:00

abstract：:Heating oncornavirus RNAs at temperatures insufficient for complete denaturation results in forms migrating between the native form (vRNA) and the completely denatured form (vRNA) after gel electrophoresis. Intermediate forms from Rous sarcoma virus or murine leukemia virus were isolated after heating of vRNA's at 58 ...

journal_title：Journal of virology

authors： Bader JP,Ray DA

更新日期：1976-09-01 00:00:00

abstract：:An active transcriptive complex was isolated from purified virions of Newcastle disease virus. After disruption with Triton X-100 and high salt, soluble and particulate fractions were separated by density gradient centrifugation. The transcriptive complex, recovered at a density of 1.275 g/cm3, appeared as a nucleocap...

journal_title：Journal of virology

authors： Colonno RJ,Stone HO

更新日期：1976-09-01 00:00:00

abstract：:Bacteriophage T4 gene 32 encodes a DNA unwinding protein required for DNA replication, repair, and recombination. Gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. ...

journal_title：Journal of virology

authors： Koch RE,McGaw MK,Drake JW

更新日期：1976-08-01 00:00:00

abstract：:Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...

journal_title：Journal of virology

authors： Kuff EL,Lueders KK,Orenstein JM,Wilson SH

更新日期：1976-08-01 00:00:00

abstract：:We have isolated a new type of temperature-sensitive mutant of simian virus 40 (SV40) that is capable of productive infection in permissive cells but not of maintenance of viral DNA integration in transformed cells at the conditional temperature. Virus development is induced when cells transformed by this mutant are s...

journal_title：Journal of virology

authors： Rothschild H,Lockwood MP

更新日期：1976-08-01 00:00:00

abstract：:Temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups I, II and IV inhibited the replication of wild-type vesicular stomatitis virus when mixed infections were carried out in BHK21 cells at 32, 37, and 39.5 C. The group IV mutant (ts G 41) was most effective in this regar...

journal_title：Journal of virology

authors： Youngner JS,Quagliana DO

更新日期：1976-07-01 00:00:00

abstract：:HeLa cell nuclei, isolated 17 h after infection with human adenovirus type 2 (Ad2), were treated with 200 mM ammonium sulfate. The extract (S200 fraction) contained 50 to 70% of the nonintegrated Ad2 DNA, which was in the form of nucleoprotein complexes. These complexes contained native, intact Ad2 DNA (with the excep...

journal_title：Journal of virology

authors： Wilhelm J,Brison O,Kedinger C,Chambon P

更新日期：1976-07-01 00:00:00

abstract：:Wild-type bacteriophage phie and IS (interference-sensitive) mutants of the related phage SP82G did not productively infect strains of Bacillus subtilis that were lysogenic for temperate phage SPO2. In these abortive infections, the sensitive phages adsorbed to and penetrated the nonpermissive host, phage-directed mac...

journal_title：Journal of virology

authors： Friedman SO,Hemphill HE

更新日期：1976-06-01 00:00:00

abstract：:T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as...

journal_title：Journal of virology

authors： Yamada Y,Nakada D

更新日期：1976-06-01 00:00:00

abstract：:The multistranded and double-stranded RNAs synthesized in HeLa cells infected with rhinovirus in the presence and in the absence of actinomycin D have been characterized by polyacrylamide gel electrophoresis and hybridization studies. The replicative form is only found in infected cells treated with actinomycin D, whe...

journal_title：Journal of virology

authors： Macnaughton MR,Cooper JA,Dimmock NJ

更新日期：1976-06-01 00:00:00

abstract：:The effect of 5-bromodeoxyuridine (BrdU) on vaccinia virus-induced polypeptide synthesis in BSC-1 cells has been investigated. Most virus-induced pre- and post-replicative polypeptides were synthesized in concentrations of 5-bromodeoxyuridine that inhibited virus growth. The synthesis of a few post-replicative polypep...

journal_title：Journal of virology

authors： Pennington TH

更新日期：1976-06-01 00:00:00

abstract：:Simian virus 40 (SV40)-transformed human cells (LN-SV) were fused with BALB/c peritoneal macrophages (BALB/c X LN-SV) and with C57BL peritoneal macrophages (C57BL X LN-SV) and hybrid clones, all of which had segregated human chromosomes and contained the entire complement of mouse chromosomes, were isolated. All 15 BA...

journal_title：Journal of virology

authors： Huebner K,Croce CM

更新日期：1976-06-01 00:00:00

abstract：:Storage of the dimeric (alphabeta) form of avian myeloblastosis virus (AMV) DNA polymerase in glycerol resulted in the release of the smaller alpha subunit, as detected by glycerol gradient sedimentation. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of enzyme stored in glycerol showed the conc...

journal_title：Journal of virology

authors： Papas TS,Marciani DJ,Samuel K,Chirikjian JG

更新日期：1976-06-01 00:00:00

abstract：:The virion RNA of Moloney murine leukemia virus (MuLV) has been translated in eukaryotic cell-free systems derived from mouse L- and human HeLa cells. In both systems at least three polypeptides, approximately 60,000, 70,000, and 180,000 in apparent molecular weight, were formed in response to the added 35S MuLV RNA. ...

journal_title：Journal of virology

authors： Kerr IM,Olshevsky U,Lodish HF,Baltimore D

更新日期：1976-05-01 00:00:00

abstract：:A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the four HgaI cleavage sites were mapped at 0.02, 0.14, 0.27, and 0.48 fractional lengths, clockwise, from the single EcoR...

journal_title：Journal of virology

authors： Shishido K,Berg P

更新日期：1976-05-01 00:00:00

abstract：:In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted trans...

journal_title：Journal of virology

authors： Hunt DM,Emerson SU,Wagner RR

更新日期：1976-05-01 00:00:00

abstract：:The nucleic acids of influenza A, B, and C viruses were compared. Susceptibility to nucleases demonstrates that influenza C virus, just as influenza A and B viruses, possesses single-stranded RNA as its genome. The base compositions of the RNAs of influenza A, B, and influenza C virus are almost identical and comparat...

journal_title：Journal of virology

authors： Ritchey MB,Palese P,Kilbourne ED

更新日期：1976-05-01 00:00:00

abstract：:Radioactive DNA complementary to nucleotide sequences in Moloney murine sarcoma virus (MSV) and Moloney leukemia virus (M-MuLV) complex was made by the endogenous reverse transcriptase reaction. These virus stocks contained a threefold excess of MSV over M-MuLV as measured by biological assay. The complementary DNA wa...

journal_title：Journal of virology

authors： Frankel AE,Neubauer RL,Fischinger PJ

更新日期：1976-05-01 00:00:00

abstract：:PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...

journal_title：Journal of virology

authors： Woodworth-Gutai M,Lebowitz J

更新日期：1976-04-01 00:00:00

abstract：:Simian virus 40 (SV40) growth on rhesus kidney cells and on the T-22 line of SV40-transformed green monkey kidney (GMK) cells is largely limited by the low plating efficiency of SV40 on these cells. In addition, a fraction of the rhesus kidney and T-22 cells are resistant to infection by SV40. Nevertheless, 72-h viral...

journal_title：Journal of virology

authors： Norkin LC,Ouellette J

更新日期：1976-04-01 00:00:00

abstract：:The mechanism whereby picornaviruses inhibit host protein synthesis while their own synthetic processes proceed unabated has remained elusive. One of our approaches to this problem was to study the ability of cell-free extracts derived from uninfected and mengovirus-infected Ehrlich ascites tumor cells to translate vi...

journal_title：Journal of virology

authors： Abreu SL,Lucas-Lenard J

更新日期：1976-04-01 00:00:00

abstract：:After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activ...

journal_title：Journal of virology

authors： Jamieson AT,Hay J,Subak-Sharpe JH

更新日期：1976-03-01 00:00:00

abstract：:Four isolates of human papovaviruses (RF, GS, DW, and MG viruses) obtained from the urine specimens of renal allograft recipients in widely separated locations were compared with BK virus. Hemagglutination inhibition tests and plaque neutralization assays showed that all were antigenically related to BK virus. All iso...

journal_title：Journal of virology

authors： Wright PJ,Bernhardt G,Major EO,di Mayorca G

更新日期：1976-03-01 00:00:00

abstract：:The replication of the single-stranded DNA (ssDNA) of parvovirus LuIII was studied in synchronized HeLa cells. After infection of the cells in early S phase, synthesis of a replicative form (RF) DNA became detectable as early as 9 h postinfection, i.e., after display of the cellular helper function(s) indispensable fo...

journal_title：Journal of virology

authors： Siegl G,Gautschi M

更新日期：1976-03-01 00:00:00

abstract：:Murine cytomegalovirus propagated in mouse embryo fibroblasts was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 x g for 90 min. (ii) The concentrated virus was passed through a Bio-Rad Bio-Gel A-15m column to eliminate contaminating materials smaller than 1...

journal_title：Journal of virology

authors： Kim KS,Sapienza VJ,Carp RI,Moon HM

更新日期：1976-03-01 00:00:00

abstract：:The role of the T4 bacteriophage regA gene in stabilizing early mRNA was investigated by assaying the level of functional mRNA from eight prereplicative genes (56 [dCMP hydroxymethylase], cd [dCMP deaminase], 1 [deoxynucleotide kinase], rIIA, rIIB, 46 [DNA arrest], and 45) during extended infection of Escherichia coli...

journal_title：Journal of virology

authors： Trimble RB,Maley

更新日期：1976-02-01 00:00:00

abstract：:The origin and role of the endonuclease activity associated with purified virions of simian virus 40, previously described by this and other laboratories, have been further investigated. We found that the enzymatic activity from virions of temperature-sensitive (ts) mutants is not more heat labile than that from wild-...

journal_title：Journal of virology

authors： Dooley DC,Ryzlak MT,Ozer HL

更新日期：1976-02-01 00:00:00

abstract：:In vivo decay rates for the individual T7 early mRNA species were determined. The physical half-lives, measured at 37 C, range from 1.1 min for gene 0.7 RNA to 4.5 min for gene 0.3 RNA. Physical half-lives, as observed after rifampin inhibition of RNA synthesis and polyacylamide electrophoresis of RNAs, are approximat...

journal_title：Journal of virology

authors： Hercules K,Jovanovich S,Sauerbrier W

更新日期：1976-02-01 00:00:00

abstract：:Several aspects of the terminal stages of T4 head maturation were investigated using ts and am mutants blocked at single steps of the assembly pathway. We had previously found that cells infected with mutants of gene 13, e.g., tsN38 and amE609, accumulated both stable (10 to 20%)- and fragile (80%)-filled head precurs...

journal_title：Journal of virology

authors： Hamilton DL,Luftig RB

更新日期：1976-02-01 00:00:00

abstract：:The binding of tail parts to the head of phage P22 is normally irreversible, but after adsorption to host cells sonication releases many of the tail parts intact. This release is dependent on the power of sonication, but is independent of the length of sonication from 2 to 32 s. This phenomenon has been used to show t...

journal_title：Journal of virology

authors： Ruddel M,Israel V

更新日期：1976-02-01 00:00:00

abstract：:The distribtuion of various amino acid tRNA's in the 4S RNA components of avian myeloblastosis virus (AMV) and in 4S RNA prepared from chicken cmbryo cells, chicken myeloblasts, and chicken livers was determined. This was done by aminoacylating the 4S RNA samples with a mixture of 17 radioactive amino acids and subseq...

journal_title：Journal of virology

authors： Waters LC,Mullin BC,Bailiff EG,Popp RA

更新日期：1975-12-01 00:00:00

abstract：:Two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of T4D and other T-even bacteriophage particles. The two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding...

journal_title：Journal of virology

authors： Kozloff LM,Lute M,Crosby LK

更新日期：1975-12-01 00:00:00

abstract：:The involvement of two bacteriophage T4 gene products in the initiation of T4 tail tube and sheath polymerization on mature baseplates has been studied by radioautography of acrylamide gels of various partially completed tail structures. The products of genes 48 and 54 (P48[the nomenclature P48 refers to the protein p...

journal_title：Journal of virology

authors： Berget PB,Warner HR

更新日期：1975-12-01 00:00:00

abstract：:Polyacrylamide gel electrophoretic analysis of purified preparations of human and calf diarrhea viruses indicated eight polypeptide components, or possibly nine in the case of the calf diarrhea virus. Thermal denaturation and analytical studies of the calf diarrhea virus genome showed it to consist of 11 double-strand...

journal_title：Journal of virology

authors： Rodger SM,Schnagl RD,Holmes IH

更新日期：1975-11-01 00:00:00

abstract：:Structural differences between human leukocyte virus-induced interferon and human fibroblast polyinosinic-polycytidylic acid (rIn-rCn)-induced interferon have been noted in previous studies. This study reports the behavior of human leukocyte and fibroblast interferon, induced by virus and by rIn-rCn, in several lectin...

journal_title：Journal of virology

authors： Jankowski WJ,Davey MW,O'Malley JA,Sulkowski E,Carter WA

更新日期：1975-11-01 00:00:00

abstract：:The glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35SO4(-2), whereas the nonglycosylated proteins are not. This was shown for the HN and F glycoproteins of SV5 and Sendai virus, the E1 and E2 glycoproteins of Sindbis virus, and for the major glycoprotein, gp69, as well ...

journal_title：Journal of virology

authors： Pinter A,Compans RW

更新日期：1975-10-01 00:00:00

abstract：:The morphology and development of four members of the reticuloendotheliosis virus group were studied by transmission electron microscopy. Virions of duck spleen necrosis virus, duck infectious anemia virus, chicken syncytial virus, and reticuloendotheliosis virus strain T are sperical with a diameter of approximately ...

journal_title：Journal of virology

authors： Kang CY,Wong TC,Holmes KV

更新日期：1975-10-01 00:00:00

abstract：:Temperature-sensitive (ts) mutants of the T4 phage rII gene were islated and used in temperature shift experiments that revelaed two different expressions for the normal rII (rII+) gene function in vivo: (i) an early expression (0 to 12 min postinfection at 30 C) that prevents restriction of T4 growth in Escherichia c...

journal_title：Journal of virology

authors： Heere LJ,Karam JD

更新日期：1975-10-01 00:00:00